cxcl2  (R&D Systems)

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Chronic stress induces pulmonary epithelial cells to produce acetylcholine that remodels lung pre-metastatic niche of breast cancer by enhancing NETosis.

doi: 10.1186/s13046-023-02836-5

Figure Lengend Snippet: Fig. 5 Chronic stress induces the chemotaxis of neutrophils into lung via CXCL2-CXCR2 axis. a. Transwell chemotaxis assay to detect the chemotaxis activity of lung culture supernatants to peripheral blood neutrophils of model mice in the control group at 1 week. CLCS: lung culture supernatant of model mice in control group; SLCS: lung culture supernatant of model mice in chronic restraint stress group at 2 weeks. b. Transwell chemotaxis assay to detect the chemotaxis activity of ACh to peripheral blood neutrophils from model mice in the control group at 1 week. c. Chemokine array to determine the contents of different chemokines in lung culture supernatants from orthotopic inoculation breast cancer mice with or without chronic restraint stress for 2 weeks. d. Transwell chemotaxis assay to examine the influence of CXCL2 neutralizing antibody on neutrophil chemotactic activity of SLCS. DMEM: culture medium to serve as the blank control; CLCS: lung culture supernatant of mice in control group; SLCS: lung culture supernatant of mice in chronic restraint stress group at 2 weeks; SLCS + iCXCL2: SLCS + 1 μg/ml CXCL2 neutralizing antibody; CXCL2: 2ng/ml. e. Cell counts of experiments as described in d. f. qRT-PCR to dynamically detect the mRNA levels of CXCR2 in lung neutrophils from breast cancer model mice with or without chronic restraint stress. ns: no sense, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: Lung culture supernatants from mice in control and stress groups, medium containing 2ng/ml recombinant murine CXCL2 (Peprotech, USA), lung culture supernatant from mice in stress group with 1 μg/ml neutrolizing anti-mouse CXCL2 (R&D Systems, USA) were added to the remaining receiver wells.

Techniques: Chemotaxis Assay, Activity Assay, Control, Quantitative RT-PCR

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Chronic stress induces pulmonary epithelial cells to produce acetylcholine that remodels lung pre-metastatic niche of breast cancer by enhancing NETosis.

doi: 10.1186/s13046-023-02836-5

Figure Lengend Snippet: Fig. 10 Schematic diagram of the mechanisms by which chronic stress remodels lung pre-metastatic niche of breast cancer. Chronic stress remodels lung pre-metastatic niche of breast cancer mainly by two ways. On one hand, chronic stress promotes production of CXCL2 chemokine in the lung, which recruits neutrophils. On the other hand, chronic stress motivates lung epithelial cells to secrete ACh that enhances NETosis of the aggregated neutrophils in the lung. The NETotic neutrophils capture the circulating tumor cells, hereby favoring lung metastasis of breast cancer. This image (AARIT33ae3) was generated by Figdraw (https://www.figdraw.com)

Article Snippet: Lung culture supernatants from mice in control and stress groups, medium containing 2ng/ml recombinant murine CXCL2 (Peprotech, USA), lung culture supernatant from mice in stress group with 1 μg/ml neutrolizing anti-mouse CXCL2 (R&D Systems, USA) were added to the remaining receiver wells.

Techniques: Generated